Plants use energy from sunlight to turn water and carbon dioxide from what they get into an energy-rich sugar called glucose. This process is called photosynthesis, which means “making things with light”. In photosynthesis, the energy in light is absorbed by photopigments.
Answer:
Human interaction with the ecosystem has rapidly spread disease to Yellowstone's wildlife, which has proven to have adverse effects on populations. Also, humans tend to leave trash in poor areas. Littering is a problem in Yellowstone because it can be ingested by the wildlife and also pollute the park.
Explanation:
Answer:
Most of the three-carbon G3P is used to make more RuBP, keeping the Calvin cycle operating. ... ATP and NADPH, which are formed during the light reactions, are both used in the Calvin cycle.
Explanation:
<h2>Differential test</h2>
Explanation:
A differential test helps to distinguish that which species lack or have the particular biochemical process,the three differential test media are:
1) Blood agar: Blood Agar is an enriched medium which is used to culture those bacteria or microbes that do not grow easily
- It is also required to detect and differentiate haemolytic bacteria, especially Streptococcus species
- It is also a differential media in allowing the detection of hemolysis (destroying the RBC) by cytolytic toxins secreted by some bacteria, such as certain strains of Bacillus, Streptococcus, Enterococcus and Staphylococcus
2) Eosin methylene blue agar (EMB): It is a selective and differential medium used to isolate fecal coliforms
- Eosin methylene blue are pH indicator dyes which combine to form a dark purple precipitate at low pH; they also serve to inhibit the growth of most Gram positive organisms
- Sucrose and lactose serve as fermentable carbohydrate sources which encourage the growth of fecal coliforms and provide a means of differentiating them
3) Mannitol Salt Agar (MSA): It is a selective and differential medium
- The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels
- Organisms from other genera may grow, but they typically grow very weakly
Three examples of tests which are done on colonies are:
1) Catalase test: Catalase is an enzyme that breaks hydrogen peroxide (H2O2) into H2O and O2
- Hydrogen peroxide is often used as a topical disinfectant in wounds, and the bubbling that is seen is due to the evolution of O2 gas
- A small amount of growth from the culture is placed onto a clean microscope slide
- Few drops of H2O2 onto the smear is added
- A positive result is the rapid evolution of O2 as evidenced by bubbling
- A negative result is no bubbles or only a few scattered bubbles
2) Indole test: The main requirement for a suitable indole test medium is that it contain
s a sufficient amount of tryptophan
- Tryptone broth is commonly used
- The tube of tryptone broth with a small amount of a pure culture is inoculated
- Incubation is done at 35°C (+/- 2°C) for 24 to 48 hours
- To test for indole production, 5 drops of Kovács reagent is directly added to the tube
- A positive indole test is indicated by the formation of a pink to red color in the reagent layer on top of the medium within seconds of adding the reagent
- If a culture is indole negative, the reagent layer will remain yellow or be slightly cloudy
3) Urease test: Urease is a constitutively expressed enzyme that hydrolyzes urea to carbon dioxide and ammonia
- The urease test identifies those organisms that are capable of hydrolyzing urea to produce ammonia and carbon dioxide
- Urease test media contain 2% urea and phenol red as a pH indicator
- An increase in pH due to the production of ammonia results in a color change from yellow (pH 6.8) to bright pink (pH 8.2)
- Urea broth is a highly buffered medium requiring large quantities of ammonia to raise the pH above 8.0 resulting in a color change
