The addition of the specific amino acids to the nascent peptide occurs after the binding of the amino acid to the tRNA via acylation of the amino acid through a process called t-RNA charging.
This charged tRNA binds to the A-site or Aminoacyl site of the ribosomes where the initiation stage of the translation begins. This amino acid is shifted to the P site or peptidyl site and exit from the E site of the ribosome.
