The addition of the specific amino acids to the nascent peptide occurs after the binding of the amino acid to the tRNA via acylation of the amino acid through a process called t-RNA charging.
This charged tRNA binds to the A-site or Aminoacyl site of the ribosomes where the initiation stage of the translation begins. This amino acid is shifted to the P site or peptidyl site and exit from the E site of the ribosome.
They may have som bad side effects such as nausea, indigestion, vomiting etc. Also the bacteria change or adapt if not taken correctly so they are no longer affected by the antibiotic.
