- synthesis of yolk proteins carried out by other non-germ cells: is panoistic ovaries, fat body elsewhere in the organism is producing proteins, carried towards and deposited in the oocyte
- labelled precursor with ferretin, when looked at under microscope it shows up black
- electrophoresis, proteins migrate along gel in an electric current, proteins in body fluid of organism were identical to the proteins within the yolk of the oocyte
- immunological methods using antibodies, more sensitive way of detecting yolk-protein-like proteins in the body fluid
This might have an evolutionary advantage as to why the egg behaves such. Presumably, this can help the yolk develop leaving it away from any inconvenient circumstance
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