The grass affects the movement by helping the water not over flow / flood
substrate is released from the active sites of an enzyme during a chemical reaction
Translation occurs in the nucleus to produce a pre-mRNA molecule. The pre-mRNA is the processed into the mature mRNA which exists the nucleus and is translated into the cytoplasm.
So, the answer is B) NUCLEUS
The isolation barriers prevents two of the species to mate, resulting in them evolving into different species