Answer:
The fragment and plasmid are both cut with the same restriction enzyme.
Explanation:
To insert a piece of DNA in a bacterial plasmid, we need to cut both plasmid and DNA insert from same regions. This is typically done by restriction enzymes or restriction endonuclease. This cutting will open the plasmid (which is circular initially) and produce <u>sticky ends.</u> Here, DNA insert can attach because of similar sequence (see attached figure). In the final step, DNA ligase will glue it in the plasmid and it will become its part. This technique has been extensively used as a DNA recombinant technology. A better representation can be seen in the attached figure where both DNA sequence of interest and plasmid are cut with the same restriction enzyme (shown as scissors) and then ligated with DNA ligase.
It’s carbon dioxide + water > glucose + oxygen + water and energy produced in the form of ATP
Attachment of acetyl groups to histone promotes transcription.
This mechanism is known as histone acetylation and it is a part of gene expression. The lysine residues inside the N-terminal tail from the histone core of the nucleosome are the substrate for enzymes-histone acetyltransferase which transfer an acetyl functional group from another molecule. Acetylation removes the positive charge on the histones and thus decrease the interaction negatively charged phosphate groups of DNA. As a result, chromatin becomes more relaxed structure and transcription is promoted.
Well, the ten biomes are:
Tropical Rainforests, Sub-tropical rainforests, savanna, tundra, arctic, boreal forests, conniferous forests, mediterrean, grassland, and desert. I'm doing this off the top of my head, but I know that Africa is mostly savanna. South America is mostly rainforest and Austrailia is mostly desert. The poles North/South are mostly frozen and tundras are the closest lands outside the arctic/anarctic circles. Other places will have a variety of biomes.
Hope this helps!
