Hey there!:
From the given data ;
Reaction volume = 1 mL , enzyme content = 10 ug ( 5 ug in 2 mg/mL )
Enzyme mol Wt = 45,000 , therefore [E]t is 10 ug/mL , this need to be express as "M" So:
[E]t in molar = g/L * mol/g
[E]t = 0.01 g/L * 1 / 45,000
[E]t = 2.22*10⁻⁷
Vmax = 0.758 umole/min/ per mL
= 758 mmole/L/min
=758000 mole/L/min => 758000 M
Therefore :
Kcat = Vmax/ [E]t
Kcat = 758000 / 2.2*10⁻⁷ M
Kcat = 3.41441 *10¹² / min
Kcat = 3.41441*10¹² / 60 per sec
Kcat = 5.7*10¹⁰ s⁻¹
Hence kcat of xyzase is 5.7*10¹⁰ s⁻¹
Hope that helps!
Proteins make up many many important structures in our cells ( ex : receptors , Enzymes , integral proteins and so on )
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Answer:</h3>
5.6 L
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Explanation:</h3>
We are given;
- Initial volume, V1 = 3.5 L
- Initial pressure, P1 = 0.8 atm
- Final pressure, P2 = 0.5 atm
We are required to calculate the final volume;
- According to Boyle's law, the volume of a fixed mass of a gas and the pressure are inversely proportional at a constant temperature.
- That is; P α 1/V
- Mathematically, P=k/V
- At two different pressure and volume;
P1V1 = P2V2
In this case;
Rearranging the formula;
V2 = P1V1 ÷ P2
= (0.8 atm × 3.5 L) ÷ 0.5 atm
= 5.6 L
Therefore, the resulting volume is 5.6 L
9 amino acids (alanine, cysteine, glycine, isoleucine, leucine, methionine, phenylalanine, proline, valine) have no hydrogen donor or acceptor atoms in their side chains.
