The volume of the rock is 150
The methane molecule undergoes oxidation and the carbon is oxidized to carbon dioxide and hydrogen to water.
In this reaction there is cleavage of four C-H bonds and two O=O bonds
there will be formation of two C=O bonds and four O-H bonds.
Overall due to more bond cleavage energy there will be evolution of energy. thus combustion is an exothermic reaction.
Answer: Contamination
Explanation: This is a known contrainst when performing transformation in the lab. Normally the Pglo plasmid asides glowing under UV, possess a Amp gene that codes for resistance to the antibiotics Ampicillin. Transform cells will survive while untransformed cells will normally die. If untransformed (non glowing, non Ampicillin resistant) cells thrive in the medium, it is definitely a case of contamination. Start again but this time, disinfect appropriately bad be cautious of any potential contamination.