"Regulation" is the biology theme among the choices given in the question that this example represents. The correct option among all the options that are given in the question is the second option or the penultimate option or option "b". I hope that this is the answer that has come to your great help.
The respiratory structure that contracts and relaxes to allow air to flow in and out of the lungs is option(c) i.e, the diaphragm.
<h3>What is
the diaphragm?</h3>
The diaphragm, a delicate skeletal muscle that separates the chest from the belly, is located at the base of the chest. When you breathe in, it flattens and shrinks. As a result, the lungs experience a vacuum effect that draws air in. The diaphragm relaxes and the air is forced out of the lungs when you exhale.
signs of diaphragm issues:
- Acid reflux, heartburn, coughing, and difficulty swallowing.
- alterations to skin tone (skin may turn blue).
- rapid heartbeat, pressure in the chest, and/or breathing difficulties (especially when lying down).
- Headaches.
- Hiccups that persist or recur frequently.
Patients who need surgery for conditions affecting the chest, including diaphragm issues, are treated by a thoracic surgeon.
To know more about diaphragm refer to: brainly.com/question/12822939
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Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
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