<span>The most crucial principle of ELISA (enzyme-linked immunosorbent assay) is a highly specific antibody-antigen interaction.</span>
ELISA is a biochemical technique used to detect the presence of an antibody or an antigen in the biological sample. Simply described, in an ELISA, an antigen is immobilized on a solid surface and then a specific antibody is applied over the surface so that it can bind to the antigen. The antibody is usually linked to an enzyme, and in the final step, a substrate for that enzyme is added. The enzyme can convert it to some detectable signal, most commonly a color change. <span>Medical usage of ELISA is in the diagnosis of HIV infection, pregnancy tests, measurement of cytokines…</span>
The client is at risk of developing urinary retention. The client has very high creatinine levels, which shows serious problems with kidney function or probably kidney damage. Tiotropium is a drug used to treat several lung diseases, such as asthma and chronic obstructive pulmonary disease. However, one of the drug's adverse effects is the retention of urine, painful urination or difficulty in urinating.
395.2kcal
Explanation:
Given parameters:
Energy required to break one C-H bond = 98.8kcals
Unknown:
Energy required to break methane = ?
Solution:
The bond dissociation energy is the heat energy absorbed in breaking a covalent bond in the gas phase to its constituents elements.
Solving
CH₄ has 4 C-H bonds
To break 1 C-H bond, 98.8kcals of energy was used,
To break 4 of the bonds; 4 x 98.8kcals will be used = 395.2kcal
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I would say that the answer is D.
In the nucleus of the cell, there are chromosomes that contain genetic material
