Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Cell reproduction is MOST applicable to growing and developing in organisms. Hope this helps!
Answer: GROW and DEVELOP
Answer:
The results are useless
Explanation:
The alkaline/alkaline results could indicate that only the protein was utilized by the organism, but it could also be a result of prolonged incubation. The organisms could have exhausted that available sugar and then reverted to protein catabolism.
Answer:Through mRNA, the DNA is able to transmit its messages out to other parts of the cell. (Learn more about mRNA here.) Information from the DNA is coded into mRNA which leaves the nucleus of the cell DNA contains the instructions needed for an organism to develop, survive and reproduce. To carry out these functions, DNA sequences must be converted into messages that can be used to produce proteins, which are the complex molecules that do most of the work in our bodies (the white region) and is used by ribosomes (outlined in green). hope this helps have a great night ❤️❤️❤️
Explanation:
Answer:
b. False
Explanation:
All biomes, even the most remote, are affected by the impact humans have on the environment.
Humans have been causing deforestation, pollution, agricultural expansion, mining, desertification, extinction of animals and plants, burning and other practices that have had extremely negative impacts on biomes around the world. In addition, all the pollution and exploitation of nature that humans have caused has caused harmful climate change that has affected even the most remote biomes.
The biome is a large set of interconnected ecosystems. Ecosystem, in turn, is an ecological system where there is life and interaction between living beings in a given space, and can vary in size, from a puddle to a large forest.
