Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
C.) <span>Plants produce proteins much differently than animals.
Hope this helps!</span>
Answer:
Burning fossil fuels moves carbon from the geosphere to the atmosphere.
Explanation:
- Fossil fuels such as coal, petroleum and natural gas are produced and stored in the geosphere. i.e. the part of the Earth that includes the Earth's interior, landforms, rocks and minerals.
- Fossil fuels, extracted from the geosphere are burned to generate energy.
- Combustion of fossil fuels releases the stored carbon into the atmosphere in the form of carbon dioxide.
