Question

using light microscopy, you examine the soil samples and the "goo" from the degraded polyurethane, will this approach allow you to observed all microorganisms present in the samples? why or why not? what are the limitations of this approach?

Answer 1

This approach will not allow you to observe all of the microorganism present in the samples. This is because you cannot look at the soil as a whole you just take some part of it that may contain some organisms while some may not be present in that sample. 

Answer 2

No, all microorganisms present in the samples cannot be observed at a time.

Further explanation

Light microscope

It is an instrument that utilizations obvious light and amplifying focal points to look at little objects which are not visible to the naked eye, or in better detail than the unaided eye permits. Amplification, in any case, isn't the most significant issue in microscopy. Negligible amplification without included detail is experimentally pointless. The handiness of any magnifying lens is that it creates preferable goals over the eye.

Process of observation under light microscope

1. First of all, place your slide on microscope.
2. Then look through the lens at sample.
3. Adjust the sample to the center where it is clearly visible.
4. Adjust the magnification of microscope.
5. Record what you see.

Limitations of light microscopy

Light magnifying instruments has lower resolution. Since the refracted light waves are spread out, the subsequent picture is obscured. Indeed, even instruments that give extra focal points to expanding amplification do next to no to improve picture goals. By using light microscope only, a small portion of sample can be observed. It cannot analyze the whole sample at a time.

Answer details

Subject: Biology

Level: College

Key words

• Light microscope
• Process of observation under light microscope
• Limitations of light microscopy

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