Hairpin like structures are formed in both DNA and RNA but are common in RNA than in DNA. This is because DNA can be double stranded or single stranded while RNA is generally single stranded structure that can be double stranded only when it forms a hair pin like structure.
The features of hairpin structure in RNA are as follows:
1. This structure is a building block of many secondary structures of RNA.
2. The termination sequence during transcription also forms a hairpin loop like structure.
3. tRNA also forms a hairpin loop like structure and helps in the process of translation.
Answer:
as a dimer consisting of two identical monomers (80 kDa subunits) that are packed together via hydrophobic interactions
Explanation:
SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis), is an electrophoretic methodology used to separate proteins that have a molecular weight between 5 to 250 kDa. SDS is a well-known ionic detergent that is able to break hydrophobic interactions and hydrogen bonds. Moreover, size-exclusion chromatography is a filtration technique that separates molecules in solution according to their molecular size. In this case, SDS-PAGE showed that the target protein is composed of two identical subunits (monomers) of 80 kDa each, which were separated by the detergent and formed one single band in the SDS-PAGE gel.
