Answer:
She left Ligase enzyme out of the mixture.
Explanation:
DNA replication is the process of formation of new molecules of DNA from the existing one. This process occurs in semiconservative way i.e each new strand consist of one newly synthesised strand and one old parent strand that act as template for the formation of new strand. replication on one strand occurs continuously from 5 prime to 3 prime but on other strands it occurs discontinuously from 5 prime to 3 prime because DNA polymerase moves only in one direction from 5 prime to 3 prime. Hence in discontinuous strands gaps remained. These gaps are filled by Ligase enzyme.
Requirements for DNA replication:
DNA replications needs
Primers
DNA Polymerase enzyme
DNA helicase enzyme
Single nucleotide binding protein
dNTPs
and Ligase enzyme.
Correct choice:
She left Ligase enzyme out of the mixture.
It may or may not turn acidic depending on the area or time of the year it is.
D. Mitosis and Cytokinesis
Answer:
Option C
Explanation:
Option
A. 6440 mL/min
B. 4340 mL
C. 3500 mL/min
D. 2940 mL/min
Solution
It is required to find the minute ventilation which is equal to the product of rate of breathing and the total amount of gas being inhaled or exhaled per breathe. Let this minute ventilation be denoted by "
"
Thus,

where TD is the tidal volume and Br is the rate of breathing
Substituting the given values we get

We will now reduce the air held in the conducting zone of respiratory system
Air held per breathe 
Total Air held

Amount of fresh air one can breathe in and out every minute

Hence, Option C is correct
Plant cells are the only cells with a cell wall.