Answer:
1.Handpicking,winnowing and sieving 2. distillation 3.distillation 5. winnowing 6.magnet
Answer:
A breakdown of the breaking buffer was first listed with its respective component and their corresponding value; then a table was made for the stock concentrations in which the volume that is being added was determined by using the formula
. It was the addition of these volumes altogether that make up the 0.25 L (i.e 250 mL) with water
Explanation:
Given data includes:
Tris= 10mM
pH = 8.0
NaCl = 150 mM
Imidazole = 300 mM
In order to make 0.25 L solution buffer ; i.e (250 mL); we have the following component.
Stock Concentration Volume to be Final Concentration
added
1 M Tris 2.5 mL 10 mM
5 M NaCl 7.5 mL 150 mM
1 M Imidazole 75 mL 300 mM
. is the formula that is used to determine the corresponding volume that is added for each stock concentration
The stock concentration of Tris ( 1 M ) is as follows:
.

The stock concentration of NaCl (5 M ) is as follows:
.

The stock concentration of Imidazole (1 M ) is as follows:
.

Hence, it is the addition of all the volumes altogether that make up 0.25L (i.e 250 mL) with water.
The grey liquid is related to the white liquids because the change in colour is the result of a chemical change/reaction between the two white liquids.
boyles law states that the volumes of a gas will decrease as pressure increases if the temperature remains constant.
charles law states that the volume of a gas will increase as temp increases if the pressure remains constant.
gay-lussacs law states that the pressure increases as temp increases if the volume remains constant.