No, the solution with bacteria in it needs to be diluted in order to be in the range of 30 – 300.
A colony-forming unit –CFU is used to estimate the number of viable (ability to divide by binary fission) microbes in a sample.
A sample of microbes is diluted and spread on a plate. Then, the number of colonies is counted, assuming that each colony have grown from a single colony-forming unit, or CFU.
Plates with more than 300 colonies are difficult to count while those with less than 30 colonies give statistically unreliable numbers of colonies to count.
When cloning by restriction digest and ligation, you use restriction enzymes to cut open a plasmid (backbone) and insert a linear fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then covalently binds the plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained in a variety of biological systems. Read on for an in-depth breakdown of how to do perform restriction digests.
Its D) UGCUGC because this sequence is the mrna if it was the dna sequence it'd be B but its definetly D
Hello,
Your answer would be:
D. stalactite; stalagmite
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