The response would become spontaneous if the value of ΔG° was negative.
According to the estimated value of ΔG°, it is shown that ΔG° value decreases as temperature value increases. The value shifts from being more favorable to being less favorable. It would appear that the value of ΔG° would be negative at a specific temperature, causing the reaction to occur spontaneously.
The reaction is in an equilibrium state if ΔG = 0. If ΔG < 0, the reaction is spontaneous in the direction written. The relationship between terms from the equilibrium is paralleled by the relevance of the sign of a change in the Gibbs free energy.
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<span>It makes a longitudinal wave because it stretches and compresses while as it slithers foward.
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Hi. You did not provide any response options. However, a PCR reaction proceeds as follows.
After the primers are added to the test tube containing the PCR components. This tube is placed in a device called a thermocycler. At that moment, the stage called denaturation will begin, where the thermocycler increases the temperature to the point of breaking the hydrogen bonds that hold the two strands of DNA together. The thermal cycler increases the temperature up to 96°C.
After that, the second step of the reaction begins. At that moment, the thermal cycler lowers the temperature to 55º - 65ºC, which is the ideal temperature for the primers to be able to attach themselves to the DNA strands, preparing them for the presence of the polymerase.
After that, the thermocycler raises the temperature to 72ºC, which is the ideal temperature for the DNA polymerase to work. At this stage, the DNA polymerase will use the DNA strand and the primer to build a new DNA strand, which will be annealed to the DNA strand used as a template.
These three steps will be repeated about 35 times, generating many copies of DNA.
liquid to gas intermolecular interaction are broken when liquid is converted to a gas.Example in CH3COOH liquid, dispersion forces, dipole-dipole forces and hydrogen bonding are intermolecular forces that must be overcomed to convert C H3COOH from liquid to gas.
