Roche/454 pyrosequencing , useful for high throughput sequencing techniques. This method utilizes pyrosequncing which allows for sequencing as the sequence read out can be achieved at the same time as the sequence is extended.
High- throughput screening is the use of automated equipment to rapidly test thousand to millions of samples for the biological activities at the model organism, cellular, pathway or molecular level.Many DNA samples can be sequenced simultaneously.
Sanger DNA sequencing also known as chain termination. This method is used only for the single DNA fragment at a time. Amplicon sequencing with NGS is efficient and cost effective . It is required a DNA polymerase , nucleotide , dideoxynucleotides and buffer.
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Answer:
The light-dependent reactions use light energy to make two molecules needed for the next stage of photosynthesis: the energy storage molecule ATP and the reduced electron carrier NADPH.
Here are the basic steps:
Light absorption in PSII. ...
ATP synthesis. ...
Light absorption in PSI. ...
NADPH formation.
Explanation:
Answer:
Carbon dioxide enters the alveoli, and oxygen enters the capillaries.
Explanation:
This describes the exchange of gases in the lungs. When blood from the rest of the body gets to the lungs through the capillaries, oxygen flows from the alveoli which are tiny air sacs in the lungs, into the blood in the capillaries.
Carbon dioxide from the blood brought to the lungs will then flow into the alveoli which will then expel it through the nose. This repeated process ensures that the body keeps getting oxygen and expelling carbon dioxide.
Answer:
The statement that is not true for antibody staining is it can provide information about gene expression.
Explanation:
Antibody staining is important aspect of applied immunology. Antibody staining is done to determine a specific protein in a given sample.Antibody staining is done by using fluorescent dyes and also by using specific enzyme such as horse reddish peroxidase,alkaline phosphatase.
Antibody staining can be visualized with either fluorescent or radioactive labels. Antibody staining can be performed by western blotting method.Multiple antibodies can be used to stain different proteins.Antibody staining requires the hybridization of the complementary base sequence of the antibody and also the target protein to which it binds.
