The first line of defence (or outside defence system) includes physical and chemical barriers that are always ready and prepared to defend the body from infection. These include your skin, tears, mucus, cilia, stomach acid, urine flow, 'friendly' bacteria and white blood cells called neutrophils.
The consumers are mostly affected secondary consumers.
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The big idea to remember is that mitosis is the simple duplication of a cell and all of its parts. It duplicates its DNA and the two new cells (daughter cells) have the same pieces and genetic code. Two identical copies come from one original. Start with one; get two that are the same. You get the idea.
So it is the very last stage!
For obtaining an adequate amount of DNA samples for DNA fingerprinting, polymerase chain reaction (PCR) can be used.
Polymerase chain reaction (PCR) is a technique that is used to amplify the DNA sample. The steps included in PCR are:-
1. Denaturation of the double-stranded DNA (at around 95°C)
2. Annealing of the primers to each of the single-stranded DNA templates ( at around 65°C)
3. Extension or elongation of the primers by considering every single-stranded DNA as a template. This elongation is carried out by <em>Taq </em>polymerase (at around 72°C).
With each cycle of PCR, the DNA sample is increased by 2ⁿ. Here, 'n' represents the number of PCR cycles.
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Learn more about polymerase chain reaction here: -
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