Answer:
1. It depends what type of method you are using. if it is Height x Width x Length then it will not work for an irregular shape because it has extra pieces that would not be included.
2. The second method would work for both regular and irregular shapes because you would have to know or find out the volume of the regular shape to get the volume for the irregular shape.
3. It also depends on what you are doing, if you are doing a regular shape then use the first method, if it's an irregular shape then use the second method, if you do the maths correctly both should give you an accurate answer for what you want to achieve.
4. No, because the sugar would dissolve.
5. No, on this case the displacement method would not work because of the weight difference
Explanation:
all the answers for you!
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
d
Explanation:
<em>The correct answer would be increasing heart rate to compensate for low cardiac output.</em>
The negative feedback loop is the mechanism that is utilized in the maintenance of homeostasis. They work by opposing the processes that bring about a deviation away from setpoints, that is, the normal range of operation of physiological processes in the body.
<u>For example, when the heart rate goes below the setpoint, the negative feedback loop swings into action through the sensor, the control center, and the effector to return the heart rate to the normal operating range.</u>
Correct option: d
