Question

One spot on the microarray contains sequences unique to a gene for an RNA polymerase subunit. One spot contains sequences unique to the skeletal muscle myosin gene. Which spot would be expected to contain the myosin gene sequences

Answer 1

Answer:

Skeletal muscle myosin gene

Explanation:

A microarray assay (also named DNA chip or biochip) is an approach used in molecular biology to detect simultaneously the expression level of hundreds of genes at the single-cell level. First, the complementary DNA (cDNA) sample is obtained from the total RNA sample. Subsequently, the cDNA is cut with restriction endonucleases and fluorophores are attached to them. Finally. the DNA fragments react with complementary known sequences (also known as probes) in order to identify by fluorescence the genes that are expressed in the sample.

Myosin is a superfamily of proteins responsible for generating the muscle contraction force both in animal and human muscle cells. In this case, the probe corresponds to the complementary sequence of the myosin gene, thereby the binding evidenced the expression of the myosin gene in the cDNA sample.