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4vir4ik [10]
4 years ago
11

Can we detect bacteria in pollutants using DNA profiling

Biology
1 answer:
Blababa [14]4 years ago
6 0
Yes. You could do this by adding solvents to break down any cell walls, centrifuge to separate the DNA, if you haven't got much then upscale with PCR, gel electrophoresis could be used to detect the DNA, and Sanger sequencing to find the sequence.  

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The PCR technique requires a DNA polymerase from an organism that can endure high heat, such as Thermus aquaticus. What step of
DENIUS [597]

Answer:

Extension

Explanation:

Polymerase chain reaction is given by Kary Mullis and it contains three steps denaturation, annealing, and extension. In denaturation, the temperature is raised to 96° c so that the DNA can denature.

In annealing, the temperature gets reduced to 55°c so that the primer can bind to the complementary sequence. In extension, Taq polymerase is used to extend the new strand.  

This enzyme is isolated from thermophilic bacteria and the temperature here is raised to 72°c so that the enzyme can extend the primer and make the complementary strand. So the right answer is extension.

5 0
3 years ago
Which would have more effect on the evolution of plants and animals?
S_A_V [24]

Answer:

E. onset of an ice age

Explanation:

8 0
2 years ago
ASAP! PLEASEEEE HELPPPPP MEEEEE NOWWW!
Nataliya [291]

Answer:

i would say 2. and 4.

tides are made by the moon..... but the plates under the Earth's suface causes larger waves.

Explanation:

i know it is not 3. but i'm not sure what 1. is.

I'm sorry i couldn't help more, but i hope this helps you out!

5 0
3 years ago
the process used by planta and other autotrophs to capture light energy and use it to power chemical reactions is called 1.) che
nalin [4]

Answer:

Photosynthesis

Explanation:

Photosynthesis is the process by which green plants use sunlight to produce their food

4 0
3 years ago
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bogdanovich [222]
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