Answer:
a. Gly-Lys + Leu-Ala-Cys-Arg + Ala-Phe
b. Glu-Ala-Phe + Gly-Ala-Tyr
Explanation:
In this case, we have to remember which peptidic bonds can break each protease:
-) <u>Trypsin</u>
It breaks selectively the peptidic bond in the carbonyl group of lysine or arginine.
-) <u>Chymotrypsin</u>
It breaks selectively the peptidic bond in the carbonyl group of phenylalanine, tryptophan, or tyrosine.
With this in mind in "peptide a", the peptidic bonds that would be broken are the ones in the <u>"Lis"</u> and <u>"Arg"</u> (See figure 1).
In "peptide b", the peptidic bond that would be broken is the one in the <u>"Phe"</u> (See figure 2). The second amino acid that can be broken is <u>tyrosine</u>, but this amino acid is placed in the <u>C terminal spot</u>, therefore will not be involved in the <u>hydrolysis</u>.
Answer: To test the properties of the particles, Thomson placed two oppositely-charged electric plates around the cathode ray. The cathode ray was deflected away from the negatively-charged electric plate and towards the positively-charged plate. This indicated that the cathode ray was composed of negatively-charged particles.
Thomson also placed two magnets on either side of the tube, and observed that this magnetic field also deflected the cathode ray. The results of these experiments helped Thomson determine the mass-to-charge ratio of the cathode ray particles, which led to a fascinating discovery−-−minusthe mass of each particle was much, much smaller than that of any known atom. Thomson repeated his experiments using different metals as electrode materials, and found that the properties of the cathode ray remained constant no matter what cathode material they originated from. From this evidence, Thomson made the following conclusions:
The cathode ray is composed of negatively-charged particles.
The particles must exist as part of the atom, since the mass of each particle is only ~1/2000 the mass of a hydrogen atom.
These subatomic particles can be found within atoms of all elements.
While controversial at first, Thomson's discoveries were gradually accepted by scientists. Eventually, his cathode ray particles were given a more familiar name: electrons. The discovery of the electron disproved the part of Dalton's atomic theory that assumed atoms were indivisible. In order to account for the existence of the electrons, an entirely new atomic model was needed.
<span>A device that generates an intense beam of coherent monochromatic light (or other electromagnetic radiation) by stimulated emission of photons from excited atoms or molecules. Lasers are used in drilling and cutting, alignment and guidance, and in surgery; the optical properties are exploited in holography, reading bar codes, and in recording and playing compact discs.</span>
According to Coulomb's Law, as the atomic number increases within a series of atoms, the nuclear attraction for electrons will also increase, thus pulling the electron(s) closer to the nucleus.
