Answer:
A) That resistance in bacteria is produced due to inactivation of ampicillin by the beta lactamase enzyme. This enzyme is expressed by the bla gene found in the plasmid. This enzyme is secreted into the culture medium, thereby inactivating ampicillin. Thanks to this inactivation, the bacteria colonies will be able to develop. After a day of incubation, only those bacteria that took the plasmid that gives them resistance to ampicillin will grow after transformation. After prolonged incubation, two types of colonies can be observed in the culture medium. One large colony with ampicillin resistance, and another small colony, both of which are sensitive to ampicillin.
B) Large colonies are characterized by being resistant to ampicillin. When Ramón isolates the plasmid, he will have the gene that provides resistance to antibiotics. Said plasmid can be used again on those bacteria that are sensitive to ampicillin.
On the other hand, satellite colonies are sensitive to ampicillin. These types of colonies do not have the plasmid that contains the gene that gives ampicillin resistance. It is not possible to isolate any plasmids from these satellite colonies. These satellite bacteria will not be able to grow if they are transferred to a plate containing fresh ampicillin, while large colonies, which possess the plasmid that gives them resistance to ampicillin, will be able to grow on that plate.
Explanation:
Answer:
d. Temperature and number of molecules of gas
Step-by-step explanation:
Boyle's Law states, "The volume of a fixed mass of a gas is inversely proportional to the pressure if the temperature remains constant."
Let's examine the words.
"… volume…is inversely proportional to the pressure …" This means that volume and pressure are the <em>variables</em>.
"… fixed mass of a gas …" means that the number of molecules is constant.
"… temperature remains constant" speaks for itself.
a, c, and e are <em>wrong</em>, because pressure is a variable.
b is <em>wrong</em>, because volume is a variable.
The volume of H₂ evolved at NTP=0.336 L
<h3>Further explanation</h3>
Reaction
Decomposition of NH₃
2NH₃ ⇒ N₂ + 3H₂
conservation mass : mass reactants=mass product
0.28 NH₃= 0.25 N₂ + 0.03 H₂
2 g H₂ = 22.4 L
so for 0.03 g :
Atomic number of C is 6. Hence, there are 6 electrons in carbon.
The electronic configuration of carbon is 1s2, 2s2, 2p2
Here, there are 2 unpaired electron. However, C2+ ions have 2 electrons less as compared to C.
Hence, electronic configuration of C 2+ ion is 1s2, 2s2. All the electrons are paired in this system. So there are no unpaired electrons in C 2+ ion.
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