Question

As a first step in the proposal, the investigator(Wouldbe Fiancé) needs to make many copies of DNA of known sequence. How would they do that? A)Carry out DNA cloning of the desired sequence into a plasmid vector and then put that into bacteria and wait for the bacteria to copy the gene every time the cells divide. B)Wait for helicase to unzip the genes. C)Make a long sequence of adenines so they could get paired with U. D)Alterthe reading frame of the mRNA.

Answer 1

Answer:

The correct option is A) Carry out DNA cloning of the desired sequence into a plasmid vector and then put that into bacteria and wait for the bacteria to copy the gene every time the cells divide.

Explanation:

The DNA can be easily cloned by the help of vectors like plasmids. Plasmids can be described as an extra-chromosomal ring which is present in bacteria. The plasmid has many multiple cloning sites where the desired DNA can be incorporated. the plasmid with the foreign DNA is then incorporated back into a bacterial cell. As bacterial cells like E.coli divide in a short period of time, many copied of our DNA could be obtained whenever the bacterial cell replicates.