Centrifugation is used to separate the suspended particles in solution.
<u>Explanation:</u>
- The method of centrifugal action commonly referred to as "Centrifugation" is majorly used to effectively separate the suspended particles from the solution by means of rotating the sample at the maximum speed.
- When the samples gets rotated at the maximum speed the suspended particles settles down at the bottom and it can be filtered using the filter paper,thus resulting in the separation of suspended particles in the solution.
- However this techniques cannot be used for the substances dissolved in solution.
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Answer:
The Michaelis‑Menten equation is given as
v₀ = Kcat X [E₀] X [S] / (Km + [S])
where,
Kcat is the experimental rate constant of the reaction; [s] is the substrate concentration and
Km is the Michaelis‑Menten constant.
Explanation:
See attached image for a detailed explanation
The ratio of buffer C₂H₃O₂ /HC₂H₃O₂ must you use are1:0.199 or 10:2
the ratio of buffer C₂H₃O₂ /HC₂H₃O₂ can be calculate using the Henderson-Hasselbalch Equation which relates the pH to the measure of acidity pKa. The equation is given as:
pH = pKa + log ([base]/[acid]
Where,
[base] = concentration of C₂H₃O₂in molarity or moles
[acid] = concentration of HC₂H₃O₂ in molarity or moles
For the sake of easy calculation, allow us to assume that:
[base] =1
[acid] = x
Therefore using equation 1,
5.44 = 4.74 + log (1 / x)
log [base / acid] = 0.7
1 / x = 5.0118
x = 0.199
The required ratio of buffer C₂H₃O₂ /HC₂H₃O₂ is 1:0.199 or 10:2
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