The rate at which an enzyme catalyses a particular reaction is calculated by the amount of substrate being used up. The concept of a chemical reaction is that the substrate is converted into product with the help of an enzyme.
Rate of reaction= Amount of substrate utilized or product formed/ Time taken
Explanation:
Temperature is an important factor in the deciding of a rate of reaction. The velocity of an enzyme <u>increases with an increase in temperature</u> until and optimum temperature is achieved. After that, the velocity of an enzyme starts <em>decreasing</em> since the enzyme starts to get denatured.
Enzymes work best at a <em>specific pH</em>. If there are changes in pH, the active site of an enzyme gets modified and the rate of reaction decreases. Certain enzymes like pepsin which is in our stomach works at an acidic pH of 2.0.
