Matter: Gases,plasma,melting &freezing, solids, vapor
Not Matter: time,sound,sunlight,heat,gravity
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Acute inferior myocardial infarction. Documented mildly occlusive plaque with much clot in the right coronary artery
<h3>
What is myocardial infarction?</h3>
- A myocardial infarction (MI), also referred to as a heart attack, happens when blood flow to the heart's coronary artery is reduced or interrupted, harming the heart muscle.
- The most typical symptom is discomfort or soreness in the chest, which may radiate to the shoulder, arm, back, neck, or jaw. It frequently lasts more than a few minutes and usually happens in the center or left side of the chest.
- Sometimes the discomfort may feel like heartburn. Other signs and symptoms could include fatigue, shortness of breath, nausea, feeling dizzy, and cold sweats.
- Atypical symptoms are present in about 30% of patients. Women more frequently experience arm, neck, or fatigue discomfort instead of chest pain.
To learn more about myocardial infarction with the given link
brainly.com/question/19884287
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