Answer:
The correct order is dehydration, embed in wax, cut into sections, staining
Explanation:
There are certain proceedings needed to obtain stained sections of vegetable or animal tissues for their microscope observations.
These steps are:
- Obtention of the material: The tissue is cut to an adequate size.
- Fixation: When tissues are extracted from the organism, they suffer autolysis and putrefaction, so they need to be fixated in order to keep their cells in the best state possible. Fixation involves preserving the original morphological and molecular characteristics of the tissue. Fixation avoids autolysis, putrefaction, distortion, and retraction of cells and the tissue that could affect its volume or morphology.
- Dehydration. Once fixated, the fixator must be eliminated and the tissue is dehydrated by using a gradual series of solutions with alcohol in ascendant concentrations. Dehydration must be gradual to avoid tissue deformation.
- Inclusion. To obtain thin cuts that can be observed under the optic microscope, the tissues must be included in a consistent, firm substance, that might be either hydrophilic or hydrophobic. A hydrophobic medium is paraffin wax, that provides hardness and plasticity.
- Cut. The tissue included in wax must be cut in slides or sections thin enough to allow the diffusion and penetration of light. A microtome is used to perform these cuts. When using paraffine for tissue inclusion, the cuts are about 5 to 20 micrometers of thickness.
- Stain. Once the cuts are performed, paraffin wax must be eliminated. This can be done by using an organic solvent. Then the tissue must be stained. Hematoxylin and Eosin are the most common dyes. Animal tissues in general do not have any natural color, so they need to be stained to be observed.
The correct answer is option A. Carrying the genetic code and determining an organism's structure and function are the functions of DNA. It is the hereditary material in almost all other organisms. The information in a DNA is stored as codes that is composed of chemical bases.
Carbohydrate residues attached to the membrane lipids are always positioned on the extracellular side of the membrane
Carbohydrates are significant parts of the cell membrane, present just on the external surface of the plasma layer, and are appended to proteins, framing glycoproteins, or lipids, and framing glycolipids. These carbohydrate chains might comprise 2-60 monosaccharide units and can be either straight or branched.
The carbohydrates of the membrane are engaged with cell bond and acknowledgment and go about as a physical barrier. Enormous, uncharged particles, for example, glucose can't diffuse through the membrane.
These carbohydrates structure particular cell markers, that permit cells to perceive one another. These markers are vital in the resistant framework, permitting safe cells to separate between body cells, which they shouldn't assault, and unfamiliar cells or tissues, which they ought to.
Learn more about membrane lipids here,
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Hi
1. Lithosphere
2. Hydrosphere
3.atmosphere
4. Biosphere
The lithosphere/geosphere is made of the earth's core, the mantle, crust, ocean floor,mountains, sand, etc.The hydrosphere is made of everything water such as oceans, rivers, lakes, rain, and snow.The atmosphere is made of gasses such as oxygen, and hydrogen.Finally the biosphere is all that has life such as plants, animals, people, insects, etc.
A more kind of fighting state, it will try new ways to attack the squirrell
