Answer:
°C. They must both be heterozygous.
Explanation: For a brown eyes parents to give birth to a blue eyes offspring it means the both parents are heterozygous.
Since D is for dominant allele and
d is for recessive allele
DD is for Homozygous individual (i.e) dominant gene
Dd is for heterozygous individual
dd is for homozygous recessive individual
Dd ×Dd- parents
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DD Dd Dd dd - offsrings 1:2:1
DD- 1 brown eye homozygous offspring
Dd- 2 brown eye heterozygous offspring
dd- 1 blue eye homozygous individual
A cross between two heterozygous individual will lead to segregation of genes in the ratio 1:2:1 i.e 3 brown and 1 blue eye individual.