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bazaltina [42]
3 years ago
10

Botox is often administered in extremely diluted form for a number of medical conditions and to reduce facial lines. It is one o

f the deadliest toxins known, causing a binding at the point where nerve endings join muscles. Several cases of poisoning have resulted from "knock-off" versions of Botox, which have been reported to be in circulation. These knock-offs have higher concentrations of the Botulinum toxin. Botox is classified under what category of toxins?
Biology
1 answer:
Burka [1]3 years ago
4 0

Answer:

it is a neurotoxic protein

Explanation:

It effects how the brain carries out daily essential life functions and hinders the medulla oblongata after long enough exposure. Also causes partial paralysis

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Please give a small paragraph quickly summarizing the what, when and how of each of the following techniques: PCR, DNA gel elect
Anuta_ua [19.1K]

Answer:

All are used to resolve questions in molecular and biochemistry/biotechnology

Explanation:

PCR: resolution of an amplicong on agarose gel to chech size after thermocycling

DNA gel electrophoresis,

Recombinant DNA, A DNA fragment that it attached to another such as a reporter, commonly used is GFP attached to protein of interest to track movement

Cloning, Duplicate an amplicon, insert into a vector, transform this vector into a bacteria that is designed to make multiple copies of itself

Northern blots, Method used to detect different sizes RNA from a mixture of other products

Southern blots, method used to detect different sizes of DNA similar to the idea of a Northern Blot

Western blots, Resolution of protein sizes by running the protein through an acrylamide gel in an ionic buffer

Antibody production (polyclonal and monoclonal),  Generating an antigen against a protein using different parts of the same protein (polyclonal) or only a specific sequence of the protein not a variety of antigens from the full length (monoclonal)

ELISA, enzyme-linked immunosorbent assay is used to check the presence of a protein

FRET, Transmission energy of one molecule to another, it is usually included in the detection of colors in fluorescence microscopy

FRAP, this method will is called Fluorescence recovery after photobleaching, a microscospy measurement

FACS, this is a type of cell sorting Fluorescence-activated cell sorting

Fractionation by centrifugation, Lysis of agents such as cells that can be lysed by spinning in a centrifuge

Chromatography, separation of chemical thru a media by colors

Fluorescence microscopy,  use of fluorophore to detect specimen under a microscope a specific wavelength

Coomassie staining,  procedure used to stain an acrylamide gel or membrane to show protein presence

Silver staining, use of a silver colloid to change the way proteins are seen on a Western blot or under a microscope

His tag, a string of histidine residues attached to a protein for easy manipulation/detection

GST tag, Glutathione S-transferases is a group of proteins used in protein purification an option other than His tag

Confocal microscopy,

FISH, The generation of a nucleotide probe used in DNA sequence detection in histology

PCR, Polymerized chain reaction used to amplify selected region of DNA

DNA library, the collection of gDNA of a specific specie or tissue

cDNA library, collection of the coding sequence of a organism/tissue

Microarrays, the platform used to detect thousands of gene sequences at once

Sanger sequencing, Method used to derrive DNA sequence developed by Frederick Sanger by incorporating a single nucleotide at a time

GFP, Green fluorescent protein, a reporter protein used in recombination protein creation

Bright field microscopy, microscopy that uses light or natural light to detect samples

DIC microscopy, Differential interference contrast is used to detect and enhance the different levels of contrast of a specimen

Phase contrast microscopy, Microscopy technique used to detect the different states of matter of a specimen

Fluorescence microscopy, use of fluorophore attached to sample for investigation

Transmission electron microscopy, uses beams of electrons to pass through a sample to then create an image

Scanning electron microscopy,  the surface of a sample is scanned with a beam of electrons to generate an image

X ray crystallography, determining a structure of a protein my using an x-ray technique

2D PAGE electrophoresis,  separation of proteins by two phases sizes and charges

NMR,  Nuclear magnetic resonance, spinning of all the nuclei and measurement of the energy that it gives off.

Mass spectrometry Protein sequencing technique based on weight

4 0
3 years ago
PLEASE HELP!
Anon25 [30]

Answer:

i think it would be france. hope this helped? hehe

Explanation:

6 0
2 years ago
What is H2O?!?!?!?!?!?!
Zina [86]

Water.

Hope this helps!

-Payshence

6 0
3 years ago
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Menopause accure in which age group​
tresset_1 [31]
Doesn’t normally happen until your 40’s or 50’s
4 0
2 years ago
True or False: The moon and sun make the day and night. Whichever we are facing makes it day or night.
Ierofanga [76]
Yes this statement is true !!
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3 years ago
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