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olchik [2.2K]
3 years ago
14

The appearance of this penguin chick with all black features might

Biology
1 answer:
d1i1m1o1n [39]3 years ago
7 0
This might represent evolution of a species or the influence of the environment on the expression of a genotype.
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A different DNA ladder from NEB (Cat #N3200L) comes as 500 uL of a 1000 ug/mL solution and is not pre-mixed with the 6 X DNA loa
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4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder

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Explanation:

DNA Ladder concentration = 1000 ug/ml

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An appropriate volume to load on an average agarose gel is 12 ul, so:

2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul

But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:

4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul

And load half of that solution (12 ul) on the gel.

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