Before the development of electrophoresis to separate macromolecules, high-speed centrifugation was used to isolate DNA.
A laboratory procedure called electrophoresis is used to divide DNA, RNA, or protein molecules according to their size and electrical charge. The molecules are moved by an electric current through a gel or other matrix. The technology of electrophoresis is crucial for the separation and examination of nucleic acids. At the lab bench, cloned DNA fragments are frequently isolated and worked with using nucleic acid electrophoresis.
High-speed centrifugation employs centrifugal force to separate particles with various densities or masses suspended in a liquid. High-speed rotation of the solution inside the tube causes each particle's angular momentum to experience centrifugal forces inversely proportionate to its mass.
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Hello!
The fission of an atom that has a large atomic number can be induced by bombarding the atom with neutrons.
Nuclear fission occurs when a heavy nucleus divides into two or more lighter nuclei. This reaction is induced by the bombarding of neutrons into the nucleus, making it unstable (like throwing an orange to a perfectly arranged orange pyramid at the supermarket). The unstable nucleus breaks down into lighter elements, releasing more neutrons which contribute to the chain reaction.
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Answer:
4.90 g
Explanation:
Given that:
volume of t-pentyl alcohol = 5 mL
the standard density of t-pentyl alcohol = 0.805 g/mL
Recall that:
density = mass(in wt) /volume
mass = density × volume
mass = 0.805 g/mL × 5 mL
mass = 4.03 g
Volume of HCl used = 12 mL
The reaction for this equation is shown in the image attached below.
From the reaction,
88.15 g of t-pentyl alcohol reacts with concentrated HCl to yield 106.59 g pf t-pentyl chloride.
4.03 g of t-pentyl alcohol forms,
of t-pentyl chloride.
Therefore,
Theoretical yield of t-pentyl chloride = 4.90 g
The factors that affect the rate of a reaction are:
- <em>nature of the reactant</em> - when reactants with different chemical composition are exposed to same conditions they would react differently. For instance, when an acid or base is added on litmus paper, blue litmus paper turns red in presence of acid while red litmus paper turns blue when base is added.
- <em>surface area</em>- a compound with small pieces spread over a large area will react faster than a big lump of a compound occupying a small area.
- <em>temperature of reaction</em>- reactants would react faster at high temperatures. this is because they have higher kinetic energy to collide with each other. Hence a plate of food on the table spoils faster than a plate of food in the fridge.
- <em>concentration</em>- an increase in concentration leads to more molecules available to collide and form products. An example, when you add more of indicator in a solution, the color becomes more clear since more particles react to give more color.
- <em>presence of a catalyst</em>- a catalyst lowers the activation energy, which means less energy is required to shift reaction in forward direction. In the presence of iron (Fe) a catalyst, nitrogen N₂ and hydrogen H₂ react to produce NH₃
