0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
Learn more about dilution at: brainly.com/question/24881505
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Answer:
5
Explanation:
According to the Law of Conservation of Matter, the mass of the products in a chemical reaction must equal the mass of the reactants.
5O2 = 10 Oxygen atoms
In 2POx there must be 10 Oxygen atoms too. => x = 5
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Hope it can help you.
These are the factor that you observe or measure.
Answer:
Kc → 5.58×10⁻⁴
Explanation:
Equilibrium reaction is:
2NOCl (g) ⇄ 2NO (g) + Cl₂(g)
Initially we have 1.25 moles of NOCl
After the equilibrium, we have 1.10 moles. So, during the process:
(1.25 mol - 1.1 mol) = 0.15 moles have reacted.
As ratio are 2:2, and 2:1, 0.15 moles of NO and (0.15 /2) = 0.075 moles of chlorine, were produced in the equilibrium.
Finally in equilibrium we have: 1.10 moles of NOCl, 0.15 moles of NO and 0.075 moles of Cl₂. But these amount are not molar, so we need molar concentration in order to determine Kc:
1.10 mol /2.50L = 0.44 M
0.15 mol / /2.50L = 0.06 M
0.075 mol /2.50L = 0.03 M
Let's make expression for Kc → [Cl₂] . [NO]² / [NOCl]²
Kc = (0.03 . 0.06²) / 0.44² → 5.58×10⁻⁴
