A mule is a hybrid between a horse and a donkey (a male horse and a female donkey). Mules cannot produce offspring so it is under Hybrid Sterility, which falls under Letter D: a postzygotic barrier.
A postzygotic barrier diminishes the ability to reproduce of hybrid offsprings.
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The blood collection centers eventually refuse to take her blood because the frequency with which she gave blood had left her anemic.
Anemia is a condition that develops when your blood lacks enough healthy red blood cells or hemoglobin.
<span>Therefore her heart must pump more blood to compensate for the lack of oxygen in the blood. The consequences are huge, leading to an enlarged heart or heart failure.</span>