Explanation:
For the detection of the antibody, several methods can be used:
Peroxidase is an enzyme that uses H2O2 to oxidize its substrates. These substrates are designed in such a way that their oxidation can be detected by light release (luminol), by the formation of a colored precipitate (DAB) or by the transformation of a colorless compound into a colorful compound, can be determined quantitatively by spectroscopy. Luminol, in the presence of H2O2 and peroxidase emits light, which can be captured by X-ray films, being mainly used in immunodetection. Diaminobanzidine (DAB), in the presence of H2O2 and peroxidase, forms a colored polymer brown, precipitating in the place where the peroxidase is found, being mainly used in immunocytochemistry studies. There is a wide variety of compounds that can be used as chromophores, providing the most varied colors, usable in ELISA technique, with ABTS (2, 2'-azino-di (3-ethylbenzthiazoline-6-sulfonate) being an example of dye that turns blue-green in the presence of peroxidase.
Alkaline phosphatase is an enzyme that hydrolyzes phosphate bonds. In this way, chromophores are compounds that have phosphate groups, and that with the loss of this group phosphates acquire color, as is the case with p-nitrophenyl phosphate, which turns yellow when phosphate group.
Fluorophore is a compound that emits light of a certain wavelength (λ (coloring) when excited by a light from another λ. Fluorophores with different characteristics make it possible to mark different antigens with different colors in the same tissue or cell, allowing simultaneous observation oftwo or more components.
- By Metals with high density
The main heavy metal conjugated to antibodies is gold. This allows you to locate antigens through electron microscopy, allowing very specific cytological studies
Absolute strength: Absolute strength: Strength measured by how much weight or resistance you can overcome regardless of your body size. It’s muscular endurance
Answer:
loss of speech
Explanation:
typically when people have heat strokes they have slurred speech. trouble talking, along with seeing a stiff or drooping face and feeling sluggish in one limb, is one of the three main symptoms of stroke. If the flow of oxygen to the brain has been cut off by a blood clot, you may have slurred voice, or be difficult to understand, or be unable to speak at all.
hope this helped!
Answer: Cobblestone
Explanation:
Crohn's disease can be defined as an inflammatory bowel disease which may affect the segment of the gastrointestinal tract that covers the mouth to the anus. This disease is characterized by the formation of lesions in the colon as well as in the bowel typically in the submucosal layer.
The submucosal layer of the bowel takes the appearance of the cobblestone due to formation of the fissures and crevices also surrounded by the submucosal edema.
