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Aliun [14]
3 years ago
12

You are adjusting pH in the fed-batch bioreactor using a 1.5 Molar solution of Ca(OH)2 (Assume that in given conditions the base

dissolves completely). In addition, a feed solution with an osmolarity of 1500 mOsm is used for fed-batch operation. The starting cell culture medium has an osmolarity of 320 mOsm. If the total base addition was 30 mL/L of culture and if the total feed was 300 mL/L of the culture, estimate the final osmolarity.
Engineering
1 answer:
mrs_skeptik [129]3 years ago
3 0

Answer:

Final osmolarity = 866 mOsm

Explanation:

Starting cell culture medium = 320 mOsm

30 mL of 1600 mM NaOH into 1 L= (30 mL/1000) x 1600 = 48 mM

Osmolarity = osmoles / 1 L = (Mole x equivalent) / L = 48 x 2 = 96 mOsm

Feed solution = (300 mL / 1000 mL) 1500 mOsm = 450 mOsm

Total osmolarity = 320 + 96 + 450 = 866 mOsm

Hope this helps!

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A 14 inch diameter pipe is decreased in diameter by 2 inches through a contraction. The pressure entering the contraction is 28
Delicious77 [7]

Answer:

5984.67N

Explanation:

A 14 inch diameter pipe is decreased in diameter by 2 inches through a contraction. The pressure entering the contraction is 28 psi and a pressure drop of 2 psi occurs through the contraction if the upstream velocity is 4.0 ft/sec. What is the magnitude of the resultant force (lbs) needed to hold the pipe in place?

from continuity equation

v1A1=v2A2

equation of continuity

v1=4ft /s=1.21m/s

d1=14 inch=.35m

d2=14-2=0.304m

A1=pi*d^2/4

0.096m^2

a2=0.0706m^2

from continuity once again

1.21*0.096=v2(0.07)

v2=1.65

force on the pipe

(p1A1- p2A2) + m(v2 – v1)

from bernoulli

p1 + ρv1^2/2 = p2 + ρv2^2/2

difference in pressure or pressure drop

p1-p2=2psi

13.789N/m^2=rho(1.65^2-1.21^2)/2

rho=21.91kg/m^3

since the pipe is cylindrical

pressure is egh

13.789=21.91*9.81*h

length of the pipe is

0.064m

AH=volume of the pipe(area *h)

the mass =rho*A*H

0.064*0.07*21.91

m=0.098kg

(193053*0.096- 179263.6* 0.07) + 0.098(1.65 – 1.21)

force =5984.67N

4 0
4 years ago
Reduce the force F ij = + (2 5 ) kN to point A(2m,3m) that acts on point B( 3m,5m) - .
Alexxx [7]

Given :

Force, \vec{F}= (2\hat{i} + 5\hat{j})\ kN.

Force is acting at point A( 2 m, 3 m ) and B( 3 m, 5 m )

To Find :

The work done by force F .

Solution :

Displacement vector between point A and B is :

\vec{d} = (3-2)\hat{i} + (5-3)\hat{j}\\\\\vec{d} = \hat{i} + 2\hat{j}

Now, we know work done is given by :

W = \vec{F}.\vec{d}\\\\W= (2\hat{i} + 5\hat{j}).(\hat{i}+\hat{2j})\\\\W = (2\times 1) +( 5\times 2) \ kJ\\\\W = 12 \ kJ

W = 12000 J

Therefore, work done by force is 12000 J .

6 0
3 years ago
Two containers initially contain dry air at 40 °C. Water is added to both containers so that the relative humidity is 30% in con
enot [183]

Answer:

A) Wet bulb temperature of #1 is less than that of #2

Explanation:

This can be gotten from pinpointing the states of the two containers on a psychometric chart.

8 0
3 years ago
Imagine yourself as an Engineer. How would you design your own Wind Turbine to generate electricity (at least 40 watts) at your
topjm [15]

Answer: You need metal and other stuff

Explanation:

6 0
2 years ago
To measure the current through a resistor, a _______________ must be placed in _______________ with the resistor.
fgiga [73]

Answer:

Explanation:

To measure the current through a resistor, the Ammeter must be placed in series with the resistor so that the same current that passes through the resistor also passes through the Ammeter. Ammeter is a measuring device used to measure current.

5 0
3 years ago
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