1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
Aliun [14]
3 years ago
12

You are adjusting pH in the fed-batch bioreactor using a 1.5 Molar solution of Ca(OH)2 (Assume that in given conditions the base

dissolves completely). In addition, a feed solution with an osmolarity of 1500 mOsm is used for fed-batch operation. The starting cell culture medium has an osmolarity of 320 mOsm. If the total base addition was 30 mL/L of culture and if the total feed was 300 mL/L of the culture, estimate the final osmolarity.
Engineering
1 answer:
mrs_skeptik [129]3 years ago
3 0

Answer:

Final osmolarity = 866 mOsm

Explanation:

Starting cell culture medium = 320 mOsm

30 mL of 1600 mM NaOH into 1 L= (30 mL/1000) x 1600 = 48 mM

Osmolarity = osmoles / 1 L = (Mole x equivalent) / L = 48 x 2 = 96 mOsm

Feed solution = (300 mL / 1000 mL) 1500 mOsm = 450 mOsm

Total osmolarity = 320 + 96 + 450 = 866 mOsm

Hope this helps!

You might be interested in
Technician A says that the connecting rod and main bearing caps should be marked before removing to ensure that they can be inst
Rama09 [41]

Answer:

Technician A

Explanation:

This is because when the dimacations are made, the surface area and length respectively can be maintained to reduce to idea of force which may lead to damage

3 0
2 years ago
The 5-kg collar has a velocity of 5 m>s to the right when it is at A. It then travels along the smooth guide. Determine its s
Gnoma [55]

Answer:

The speed at point B is 5.33 m/s

The normal force at point B is 694 N

Explanation:

The length of the spring when the collar is in point A is equal to:

lA=\sqrt{0.2^{2}+0.2^{2}  }=0.2\sqrt{2}m

The length in point B is:

lB=0.2+0.2=0.4 m

The equation of conservation of energy is:

(Tc+Ts+Vc+Vs)_{A}=(Tc+Ts+Vc+Vs)_{B} (eq. 1)

Where in point A: Tc = 1/2 mcVA^2, Ts=0, Vc=mcghA, Vs=1/2k(lA-lul)^2

in point B: Ts=0, Vc=0, Tc = 1/2 mcVB^2, Vs=1/2k(lB-lul)^2

Replacing in eq. 1:

\frac{1}{2}m_{c}v_{A}^{2}+0+m_{c}gh_{A}+      \frac{1}{2}k(l_{A}-l_{ul})  ^{2}=\frac{1}{2}m_{c}v_{B}^{2}+0+0+\frac{1}{2}k(l_{B}-l_{ul})  ^{2}

Replacing values and clearing vB:

vB = 5.33 m/s

The balance forces acting in point B is:

Fc-NB-Fs=0

\frac{m_{C}v_{B}^{2}   }{R}-N_{B}-k(l_{B}-l_{ul})=0

Replacing values and clearing NB:

NB = 694 N

6 0
4 years ago
Read 2 more answers
If an elevator repairer observes that cables begin to fray after 15 years, what process might he or she use to create a maintena
otez555 [7]

Answer:

inductive reasoning

Explanation:

Inductive reasoning is one of the type of reasoning method in which generalized consequences are derived from limited observations. By observing few data, general conclusions are drawn. The conclusions drawn are false in inductive reasoning. In the given situation, the conclusion drawn by the elevator repairer has been drawn by inductive reasoning. His observation of some cables led him to draw the conclusion about all the cables. The result of the reasoning is false.

5 0
3 years ago
Please calculate the current for the circuit below
Aleks04 [339]

Answer:

The answer is "I = 0.0085106383 \ A"

Explanation:

Given:

R= 470  \ \Omega \\\\V= 4 \ v

Formula:

\to V=IR\\\\\to I = \frac{V}{R}\\\\

      = \frac{4}{470}\\\\ = 0.0085106383 \ A

3 0
3 years ago
I really need some help here!
Virty [35]

Explanation:

<h3>1.*Frame Structure have joins which keep them together whereas Shell Structure have no joins.</h3><h3>*Shell Structure usually only support their own weight whereas Frame Structure support others weight as well as themselve.</h3>
7 0
3 years ago
Other questions:
  • Discuss the effect of PRE and CLR in JK flip-flop.​
    8·1 answer
  • You are using a Geiger counter to measure the activity of a radioactive substance over the course of several minutes. If the rea
    8·1 answer
  • Hi. I would like to know why one side of an island can get more rain (more rain forms), while the other gets less.
    15·2 answers
  • Which type of voltage do generators produce?
    6·2 answers
  • You talk with the owner and he likes the idea of using two large glulam beams as shown to carry the joist loads. Design the glul
    5·1 answer
  • Consider a 6-bit cyclic redundancy check (CRC) generator, G = 100101, and suppose that D = 1000100100. 1. What is the value of R
    9·1 answer
  • Explain the difference between the connection of a cumulative compound and a differential compound motor
    10·1 answer
  • Select the correct answer. which process involves creating a product by heating metals and changing their shape through the appl
    13·1 answer
  • What is an advantage of using a fully integrated cloud-based data analytics platform?
    14·1 answer
  • the water level in a tank is 20 m above the ground. a hose is connected to the bottom of the tank, and the nozzle at the end of
    5·1 answer
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!