Hi
Below are five major steps of DNA or gene cloning:
1: You chose the gene or specific piece of DNA you need to clone and cut the gene with restriction enzymes from the source organism.
2: You need to choose a vector for the process of cloning and you will cut the vector with same restriction enzymes through which you have cut your target DNA sequence to be inserted into the vector.
3: You place the target gene into the vector and join or seal the gene with vector by using an enzyme called DNA ligase.
4: You introduce the vector with your target gene into a suitable host organism such as yeast or bacteria through the process of Transformation. In this process host organism takes up the vector containing your target gene and starts replicating the target DNA along with their own DNA and thus creating millions of copies of target gene .
5: In the last step, the DNA or target gene is isolated from host organism and purified and is ready to be used since its quantity has been enormously increased through the process of cloning.
The cloning is also called as recombinant DNA technology and is the main process that is being used in the production of insulin for diabetes patients. You can see below image for better understanding.
Hope it help!
Answer:
HE IMPROVED THE WORLD BY GIVING SOMTHING THAT IT DID NOT HAVVE
Explanation:
ENJOY :)
Answer:
Power has to travel a long distance over power lines to reach cities. Because there is no power plants.
Explanation:
Please tell me if I am wrong! good luck!
<span>The ash trees are being cut when the show signs of
infestation of the ash borer. Ash borer in particular the Emerald Ash Borer
(EAB) is a tiny bug but can really do a great damage to the tree. When the tree
is infested with the ash borer, they lay their eggs in the bark of tree, when
the eggs hatch the lavas would start eating on the inner tissues of the tree
blocking the flow of nutrients and water in the tree that causes the tree’s to
death. If the tree is infested they cut
it down, burn and bury the wood to stop the spread of infestation. Ash trees
are valued for its strength and elasticity that is often used to make baseball
bats, bows, tool handles and other products that needs durable wood.</span>
Answer:
Explanation:
Plants infected with white rot may have a cottony white or grey mass of fungal ... The disease can occur on all the vegetable alliums but tends to be more serious on garlic ... The fungus is an important pathogen on a number of non-allium crops ... a brown discoloration and rot, so that the shoot easily detaches from the base.