Question

You have tested an unknown sample using both theBenedict’s and Biuret tests. The solution mixed with the biuret reagent is blueafter 2 minutes. The solution mixed with Benedict’s is also blueafter being boiled for five minutes. What can you conclude about the sample based on these results?

Answer 1

Answer:

The sample contains protein.

Explanation:

Biuret test is a test used to determine or assay for proteins in a solution. When the biuret reagent is added to a solution and it turns blue after 2 minutes, it means that the solution contains protein.

Benedict’s test is used to determine the presence of sugar (reducing sugar) in a solution. When it turns brick red, it means there is the presence of reducing sugar in large quantity.

From the question, since the solution turned blue on addition of Biuret  reagent and was also blue on addition of Benedict’s reagent (the reagent is usually blue), it means that the solution contained just proteins excluding reducing sugars.