The enzyme Taq polymerase is required in the PCR process because it is the main enzyme that synthesizes the new DNA strands complementary to the template strand and without the activity of this enzyme the PCR will be useless and it cannot make the copy of the given strand in any way. Thus, this enzyme is very much necessary for the efficient working of the PCR.
Polymerase Chain Reaction is a very efficient technique that can able to detect and copy even a small amount of DNA by performing a set of reactions at different temperatures.
The PCR process begins with the denaturing of the template strand at 95°C, followed by annealing in which the appropriate primers bind to the single strands of DNA at 50-56°C, followed by extension at 72°C in which the thermostable Taq polymerase synthesizes the strand complementary to the template DNA.
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The answer is template strand; daughter strand.
<span>In DNA replication, each individual parent strand acts as a <u>template</u> strand for the synthesis of a <u>daughter</u> strand. DNA replication is a process of producing of two identical copies of DNA from the original DNA. DNA molecule consists of two strands and each strand serves as a template strand for the synthesis of a daughter cell. DNA polymerase binds to the origins of replication and synthesises daughter strands by adding complementary nucleotides.</span>
<u>Answer</u>:
1. Type of medium - the nutrient availability is one of the vital factors for the growth of a bacterial colony. Thus, the used growth medium will fulfill this requirement.
2. Incubation temperature - all bacteria have an optimal temperature range in which maximum growth is achieved. Any variations from this will result in negative effects on the amount of bacteria that are produced.
3. Moisture - bacteria thrive in moist or wet environments. Thus, the availability of moisture is another vital factor for the growth of the colony.
Answer:
the answer is always wear safety goggles