Denaturation of DNA is a process in which DNA loses its structure (secondary, tertiary, quaternary). It is usually achieved via the use of high temperature, strong acid or base, concentrated salt etc. The denaturation of the double-stranded DNA is used in Southern blotting procedure so that single strands can bind to the membrane and become ready for hybridization.
Water molecules will move from the side of higher concentration to the side de of lower concentration until both solutions are isotonic at this point the equilibrium will be reached.
