Question

For some RNA species, this can be accomplished via affinity chromatography, using beads coated with chains of poly-deoxythymidine (poly-dT). The desired RNA will stick to the beads while unwanted RNAs will flow through the column. The retained RNA can then be eluted.
What RNA species can be purified using this method?

A. bacterial mRNA
B. bacterial rRNA
C. eukaryotic rRNA
D. eukaryotic mRNA

Answer 1

Answer:

Option D, eukaryotic mRNA

Explanation:

Chromatography is a biophysical technique which is used for analyzing mixture both qualitatively and quantitatively

Also the components of the mixture can be separated, identified, and purified.

Generally, a mobile phase move in contact with stationary phase through a selective absorbent. Molecules are recognized and retained based on the different extent of force applied in different degrees between atoms. Substances in the mobile phase have binding site for the immobilized substrate bind. Eukaryotic mRNAs has  5’ m7G cap structure and 3’ poly (A) tail and hence as the eukaryotic mRNA complex i.e 4F complex combines with the 5’ m7G cap structure its translation starts and it becomes easy to identify it

Hence, option D is correct