Answer:
1.protons
2.Down
Explanation:
The ATP Synthase is an enzyme which catalyses the phosphorylation ADP to ATP, for synthesis of ATPs in the matrix of mitochondria and stroma of chloroplast.
ADP + Pi → ATPs.
ATPsynthase.
The source of energy to power the ATP synthase is from the electrochemical gradient set up by diffusion of protons from the intramembrane layer of mitochondria and thylakoid membrane,down into the matrix or stroma where the ATPase synthase is located.
The force for the proton pump into the intramembrane is known as the Proton Motive Force.(PMF).it is set up by the electrical mechanisms of electron transport chain. It constantly pumps protons into the intramembrane layers, therefore setting up the electrochemical gradient.
The proton is obtained from the splitting of hydrogen atoms, carried by the co-factors NADH+ and FADH2,from the Krebs cycle into the matrix or stroma, into protons and electron.
H2→→→ H+ + e-.
This explains the ATP synthase activities for ATP synthesis.