Question

Suppose that you want to purify a PCR product after gel electrophoresis. You decide to weigh an empty microcentrifuge and the wight is 1.359 g after you add the gel cut out the weight increases to 1.895 g. Based on this information what would be the volume of the QG buffer added in order to help dissolve the gel?
Select one:

a. 1.25 ml
b. 536 µl
c. 1.0 ml
d. 136 µl
e. 1.6 ml
f. 1.4 ml

Answer 1

Answer:

e. 1.6 ml

Explanation:

Gel cut weight =  Weight before addition of gel - Weight after addition of gel               = 1.895 g - 1.359 g

= 0.536 g

You need to add 3 volumes of QG buffer to 1 volume of Gel cut PCR product to completely dissolve the DNA.

Volume of QG buffer = 0.536 g X 3

                                   = 1.6 g (1 g is approximately 1 ml)

                                   = 1.6 ml