Answer: A double restriction digest
Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no
Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.
Answer:
The correct answer is "5-1-3-2-4".
Explanation:
Internalization of LDL particles into cells, is needed to form the intracellular vesicles known as endosomes. The order of events that allow for this process are:
5) LDL receptors migrate to the cell surface and cluster in clathrin-coated pits. Clathrin acts directing the receptors to the cell membrane region where endosomes are formed.
1) A combination of cholesterol and apolipoprotein binds to LDL receptors and becomes internalized as endocytotic vesicles. Once the receptors are in the proper cell membrane region, cholesterol and apolipoprotein are bound and internalized.
3) Several endocytotic vesicles fuse to form an endosome.
2) The environment of the endosome becomes acidic, which causes the LDL to dissociate from its receptor; additionally, the endosome fuses with a lysosome. LDL should be dissociated from its receptor since it is going to be degraded in the following step.
4) The LDL particle is degraded by the lysosome. This takes place after endocytosis, when LDL particles are transported into lysosomes once they are fused, cleaving the cholesterol esters into cholesterol and fatty acids.
The answer is <span>C. DNA controls the production of protein in the cell.
DNA is made up of nucleotides. The sequences of nucleotides carry information for the protein synthesis. DNA is stored inside the nucleus, one of the organelles present in all eukaryotic cells.</span>
36 glasses is 27 Liters; 8 ounce glass= 1/4 of a liter