While the absorbance at 420 nm is related to the amount of o-nitrophenol produced, the absorbance at 600 nm is proportional to cell density, which aids in standardizing our estimations of enzyme activity.
<h3>Describe absorbance.</h3>
The amount of light absorbed by a solution is measured by its absorbance (A), often referred to as optical density (OD). The amount of light that may flow through a solution is called its transmittance.
<h3>How is the activity of beta-galactosidase determined?</h3>
The colorless ONPG substrate is changed by beta-Galactosidase into galactose and the chromophore o-nitrophenol, which results in a vivid yellow solution. The amount of substrate transformed at 420 nm can be calculated by measuring the solution's beta-galactosidase activity using a spectrophotometer or a microplate reader.
<h3>What is measured by the beta-galactosidase assay?</h3>
The -Gal Assay Kit gives users the tools they need to swiftly assess the amounts of active beta-galactosidase expressed in cells that have been transfected with plasmids encoding the lacZ gene.
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Answer:
Nuclear DNA is a pieces of genetic information tend to be passed down from
Answer: :Total distance to be travelled = 4.4 km
= 4400 m
Diameter of wheel = 70 cm = 0.7 m
Radius of wheel= 0.7 / 2 m
Circumference of wheel = 2 * pi *r
= 2* 22*0.7 / 7* 2
= 22*0.1
= 2.2 m
No. Of revolutions = Total distance / Circumference of wheel
= 4400 / 2.2
= 2000
Explanation:
Answer : cathode, protocathode
During an electrochemical reaction, electrons move through the external wire toward the cathode and positive ions in the salt bridge move toward the protocathode.
Because they can't hold it them selves.
