Question

The protein pictured is bovine insulin. Determine the residue number and the size of the fragments that would be generated upon treatment with following proteases (1) trypsin, (2), chymotrypsin and (3) BrCN; (a) without DTT and (b) with DTT. (Where DTT breaks disulfide bonds).

Answer 1

Answer:

The residue number and size of the fragments that would be generated upon treatment with proteases should be as follows:

Explanation:

1. Without DTT:

• Trypsin 1, 7 and 43 amino acids.

• Chymotrypsin 1, 1, 1, 4, 5, 10, 29.

• BrCN 51

2. With DTT:

• Trypsin 1,7, 21 and 22 amino acids
• Chymotrypsin 1,1, 1, 2, 4, 5, 8, 14, 15.
• BrCN 21 and 30 amino acids