Answer:
In acid-fast staining, carbon fuchsin is used as a primary stain which dissolves the mycolic acid present in the cell wall of <em>Mycobacterium smegmatis </em>and penetrates through it which results in staining <em>Mycobacterium</em> red.
Staphylococcus aureus cell wall does not contain mycolic acid so carbon fuchsin does not penetrate its cell wall, therefore, it becomes colorless after destaining with acid alcohol.
After destaining step methylene blue is added to stain non-acid-fast bacteria blue. So if I mistakenly forgot to use methylene blue during the procedure <em>Mycobacterium smegmatis</em> will appear red due to carbon fuchsin present in their cell wall and S<em>taphylococcus aureus</em> will appear colorless because it is destained.
Answer:
The correct answer is: Gain-of-function mutation.
Explanation:
- Mutation can be defined as the sudden alteration in the sequence of DNA or Deoxyribonucleic Acid, which if happens in the protein-coding region of the gene can cause the protein to become non-functional or can change the function of the protein.
- If such a mutation happens in the untranslated region or the regulatory sequence of the gene, it can cause:
- the gene to get constitutively (all the time) expressed at high levels, such that its expression can no longer be regulated or controlled. This type of mutation is called a Gain-of-function mutation.
- the expression of the gene to get repressed or suppressed, such that very less amount of protein or no protein that is encoded by the gene is produced. This type of mutation is called a Loss-of-function mutation.
Find your final sum by adding together the two single digits that you've added to each number, and subtracting it from your initial sum. ...
<span>Eukarya arose from the first prokaryotic organisms more than 1.7 billion years ago.</span>