Answer:
CCAGGCC
CCATCGA
GGCCATC
CAT
AGGCCAT
CATCGAG
Explanation:
Shotgun sequencing is a method used to determine the nucleotide sequence of entire chromosomes/genomes. This sequencing method consists of obtaining random DNA fragments which are subsequently classified by bioinformatic tools that ordering them according to overlapping sequences called contigs. In the whole-genome shotgun (WGS) technique, the entire genome of an organism is sequenced, being the critical factor the depth of sequencing, which refers to the quality of the sequencing reads (e.g., a depth of 20X indicates that the genome is sequenced 20 times by a sequencing machine). For the human genome, WGS became available after the completion of the Human Genome Project (HGP), which enabled the generation of a reference sequence for the whole human genome. The steps of the WGS technique are the following:
1. Preparation of isolated chromosomes
2. The DNA is sheared into small fragments
3. The DNA fragments of about 1 kilobase (1000 base pairs) are incorporated into plasmids which are cloned to render pure samples of each DNA fragment
4. The plasmid clones are sequenced by sequencing machines
5. Bioinformatic tools finally are used to link DNA fragments by their overlapping ends
Answer:
See the answer below
Explanation:
1. The data from the experiment shows that <u>as the percentage of salt in the solution used to water the plants increased, the growth rate of the plants decreased</u>. As the percentage increased from A to D, the change between the average initial and the final heights decreased, meaning that an increase in the percentage of the salt in the solution used to water the plants had a negative impact on the growth rates of the plants.
2. Due to the solutions used to water the plants in groups E and F being more concentrated (hypertonic) to that of the cell sap of the plant, <u>water molecules diffused out of the cell sap to the ambient solution</u>. As time goes by, the plants lose too much water, become wilted, and died off eventually. Instead of water molecules to keep diffusing into the plants in order for them to grow, the reverse was occurring and this led to their death.
Answer:
c. population
Explanation:
biosphere includes different species and a broad ecosystem
ecosystem is different species
environment refers to a just the type of ecosystem such as aquatic environment
Answer:
The correct answer is - both pathways glycolysis pathway and gluconeogenesis pathway.
Explanation:
The enzyme triose phosphate isomerase includes the Glyceraldehyde 3 P dehydrogenase. It is one of the enzymes used in glycolysis as well as gluconeogenesis. These enzymes catalyze the conversion of glyceraldehyde to 1,3 bis phosphoglycerate.
Thus, the correct answer is - both pathways glycolysis pathway and gluconeogenesis pathway.