I believe the answer is inertia.
I hope that was helpful.
Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
Option A
Explanation:
Thyroid is primarily caused by lack of iodine but there are other reasons as well that lead to increased size of the thyroid gland. Some of these reason is
a) over- or underproduction of thyroid hormones
b) Nodules in the glans
Some times, due to overstimulation immune system itself attack the thyroid gland thereby producing excess thyroxine and hence causing the thyroid to swell
Thus, Option A is correct
According to the graph, the times for high tide would be 1 AM and 1 PM.
The low tides would be at 7 AM and 8 PM.
